منابع مشابه
Brain Glutamate Decarboxylase
Glutamate decarboxylase (glutamate 1-carboxylyase, EC 4.1.1.15, GAD)* IS the major, rate-limiting enzyme in brain for synthesizing gamma-aminobutyric acid (GABA). Total GAD activity in brain is 10-20 times greater than the observed rate of GABA synthesis (Collins, 1972; Matsui and Deguchi, 1977; Casu and Gale, 1981), indicating that GAD operates at only a fraction of its capacity. Although othe...
متن کاملBrain L-glutamate decarboxylase: purification and subunit structure.
Glutamate decarboxylase (GDCase; L-glutamate-1-carboxy-lyase, EC 4.1.1.15) was purified from whole rat brain approximately equal to 1300-fold to apparent homogeneity with a specific activity of 2.4 units per mg of protein by a combination of column chromatographies on DEAE-cellulose, hydroxylapatite, and gel filtration, and preparative nondenaturing polyacrylamide gel electrophoresis. The purif...
متن کاملPartial Cloning and Nucleotide Sequencing of Glutamate Decarboxylase Gene Isoform 65 from Human Brain
Background: Gamma -aminobutyric acid (GABA), a non-protein amino acid acts as an inhibitory neurotransmitter in the central nervous system of mammalians. The glutamate decarboxylase (GAD) is responsible for the conversion of L-glutamate to GABA. The human brain has two isoforms of this enzyme, GAD65 and GAD67 that differ in molecular weight, amino acid sequence, antigenicity, cellular location ...
متن کاملpartial cloning and nucleotide sequencing of glutamate decarboxylase gene isoform 65 from human brain
background: gamma -aminobutyric acid (gaba), a non-protein amino acid acts as an inhibitory neurotransmitter in the central nervous system of mammalians. the glutamate decarboxylase (gad) is responsible for the conversion of l-glutamate to gaba. the human brain has two isoforms of this enzyme, gad65 and gad67 that differ in molecular weight, amino acid sequence, antigenicity, cellular location ...
متن کاملA membrane form of brain L-glutamate decarboxylase: Identification, isolation, and its relation to insulin-dependent diabetes mellitus
A membrane form of L-glutamate decarboxylase (GAD) was identified and purified to apparent homogeneity from hog brain. The purified GAD was established as an integral membrane protein by phase-partitioning assay, charge-shift electrophoresis, and chromatography on a hydrophobic interaction column. This membrane GAD has a native molecular mass of 96 ± 5 kDa and is a homodimer of 48 ± 3-kDa subun...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1995
ISSN: 0021-9258
DOI: 10.1074/jbc.270.12.6464